A New Electrochemical Method for Simultaneous Determination of Acyclovir and Methotrexate in Pharmaceutical and Human Plasma Samples

Recent clinical and pre-clinical data demonstrate that adjuvant antimicrobial therapy is beneficial in cancer treatment. For the first time, an electrochemical method was proposed for the simultaneous determination of acyclovir (ACV) and methotrexate (MTX) at activated or electropretreated pencil graphite electrode (EPPEG). Their simultaneous determination was achieved by cyclic voltammetry (CV) and adsorptive square wave voltammetry (AdSWV) techniques. The proposed sensor has a wide linear range of 2×10 -7 to 1.4×10 -6 M for MTX and 5×10 -7 to 3×10 -6 M for ACV. The limits of detection (LOD) values were found 1.13×10-8 M and 6.07×10-8 M for MTX and ACV, respectively. The proposed method was applied in their pharmaceutical formulations and human plasma. In addition the proposed method could be applied in pharmaceutical laboratories and quality control analysis in the near future. KeywordsAcyclovir, Methotrexate, Adsorptive square wave voltammetry, Electropretreated pencil graphite electrode, Human plasma